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Scaffolds Designing from Protein-loadable Coaxial Electrospun Fibermats of poly(acrylamide)-Co-poly(diacetone acrylamide) and Gelatin

[ Vol. 4 , Issue. 2 ]

Author(s):

Yuji Tanikawa, Akiko Obata, Kenji Nagata, Toshihiro Kasuga and Toshihisa Mizuno*   Pages 84 - 92 ( 9 )

Abstract:


Background: Aiming at in situ regenerative therapy, the tailored design of cytokine-releasing scaffolds is still one of the crucial issues to be studied. A core-shell fibermat is one of the attractive platforms for this purpose. But, very few detail the importance of choosing the right material for the shell units that can endow efficient release properties.

Objective: In this study, we characterized the effectiveness of core-shell fibermats that possess cross-linked gelatin (CLG) as the shell layer of constituent nanofibers, as a protein-releasing cell-incubation scaffold.

Methods: For the core nanofibers in the core-shell fibermats, we utilized a crosslinked copolymer of poly(acrylamide)-co-poly(diacetone acrylamide) (poly(AM/DAAM)) and adipic acid dihydrazide (ADH), poly(AM/DAAM)/ADH. By coaxial electrospinning and the subsequent crosslinking of the gelatin layer, we successfully constructed core-shell fibermats consisting of double-layered nanofibers of poly(AM/DAAM)/ADH and CLG. Using fluorescein isothiocyanate-labeled lysozyme (FITC-Lys) as a dummy guest protein, we characterized the release behavior of the coreshell fibermats containing a CLG layer. Upon loading basic fibroblast growth factor (bFGF) as cargo in our fibermats, we also characterized impacts of the released bFGF on proliferation of the incubated cells thereon.

Results: Although the single-layered poly(AM/DAAM)/ADH nanofiber fibermats did not adhere to the mammalian cells, the core-shell fibermat with the CLG shell layer exhibited good adherence and subsequent proliferation. A sustained release of the preloaded FITC-Lys over 24 days without any burst release was observed, and the cumulative amount of released protein reached over 65% after 24 days. Upon loading bFGF in our fibermats, we succeeded in promoting cell proliferation, and highlighting its potential for use in therapeutic applications.

Conclusion: We successfully confirmed that core-shell fibermats with a CLG shell layer around the constituent nanofibers, were effective as protein-releasing cell-incubation scaffolds.

Keywords:

Fibermat, co-axial electrospinning, protein-encapsulation, gelatin, scaffold, growth factor, CLG shell layer.

Affiliation:

Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-Cho, Showa-Ku, Nagoya, Aichi 466-8555, Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-Cho, Showa-Ku, Nagoya, Aichi 466-8555, Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-Cho, Showa-Ku, Nagoya, Aichi 466-8555, Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-Cho, Showa-Ku, Nagoya, Aichi 466-8555, Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-Cho, Showa-Ku, Nagoya, Aichi 466-8555

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